Farhan Al-Behadili; Qasim Ahmed; Thamer Al-Shuwaili; Beibei Li; Yonglin Ren
Volume 24, Issue 2 , June 2024, , Pages 1-13
Abstract
The hypothesized role of stress-inducible heat shock proteins is to act as a buffer against environmental variations and affect fitness in suboptimal conditions. Our study examining the functions of heat shock protein 27 (Hsp27) in the eggs of the Mediterranean fruit fly (Ceratitis capitata). We utilized ...
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The hypothesized role of stress-inducible heat shock proteins is to act as a buffer against environmental variations and affect fitness in suboptimal conditions. Our study examining the functions of heat shock protein 27 (Hsp27) in the eggs of the Mediterranean fruit fly (Ceratitis capitata). We utilized double-stranded RNA, specifically targeting the Hsp27 gene, to reduce its expression and assessed the consequent impact on egg viability. The investigation included the examination of early eggs (less than 6 hours post-laying) and late eggs (more than 42 hours post-laying), using varying concentrations (0.02, 0.1, and 0.2 µg/µL) of Hsp27 dsRNA. Control groups of eggs were dsRNA of ATPase, Cctra-2, or phosphate buffer solution (ph-B-S). Down-regulating Hsp27 dsRNA reduced the hatching rate of the eggs compared to the hatching rate in the control groups, especially in the ph-B-S group. The early eggs were more affected than the late eggs after soaking with Hsp27 dsRNA, where the Hsp27 dsRNA decreased the hatching rates to 12.91% early. The tested concentration of 0.2 µg/µL of Hsp27 dsRNA was achieved with the most significant reduction of 2.75 in egg viability. The sex ratio of insects hatched from treated eggs was investigated after hatching. After the down-regulation of Hsp27, there was no significant effect of the Hsp27 down-regulation on the sex ratio.
Safa Zakaria Bakr; Abdulkareem Hassan; Haider Ali Reda
Volume 22, Issue 2 , June 2022, , Pages 111-119
Abstract
A field study was conducted in date palm orchards in Salah al-Din Governorate during the period from 1/10/ 2020 to 30/10/2021 to survey the species of date palm borers and estimate their population density and relative presence during the study period. The results showed the diagnosis of three species ...
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A field study was conducted in date palm orchards in Salah al-Din Governorate during the period from 1/10/ 2020 to 30/10/2021 to survey the species of date palm borers and estimate their population density and relative presence during the study period. The results showed the diagnosis of three species of palm borers belonging to Coleoptera of the family Scarabaeidae, which included Oryctes elegans, Pentodon sp and Tanyproctus sp. The relative presence of males and females for the three borers was (53.00, 47.00%), (51.98, 48.02%) and (52.76, 47.24 %), respectively., the highest population density for males and females of the three borers during the month of June was (224, 192), (145, 132) and (68, 57) respectively, and the sex ratio for each of the three borers was 1.1:1 The highest total of adults caught by IPM Russell traps during one year was for O. elegans and Pentodon sp. it reached 418 and 236.3 adults/trap, respectively, while the total of adults of the two insects in the local trap was 75.7 and 49.9 adults/trap, respectively. For the borer Tanyproctus sp., the local trap superior on other traps , in which the total number of adults caught was 312 adults/trap, while it was 50 adults/IPM Russell trap.