Ahmed Khalid; Nagam Khudhair; Jacob Njaramba Ngatia; Le Zhang; Yan Chun Xu
Volume 24, Issue 1 , March 2024, , Pages 148-155
Abstract
Standardized methods for fecal sample collection and safe long-distance transportation for DNA extraction are yet to be identified. We compared four different preservation methods for bird fecal samples: storage in 75% and 100% ethanol, freezing at −20°C, and immersing in 100% ethanol for 3 weeks ...
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Standardized methods for fecal sample collection and safe long-distance transportation for DNA extraction are yet to be identified. We compared four different preservation methods for bird fecal samples: storage in 75% and 100% ethanol, freezing at −20°C, and immersing in 100% ethanol for 3 weeks followed by drying the samples for more than 60 days and transporting them to another country. Our objectives were to quantify the DNA concentration and amplify a fragment of the gene from each sample successfully using the primers mcb398 and mcb869 through DNA barcoding. Our data showed that the method of sample preservation used affected the DNA concentration and amplification. The best results were achieved when the samples were preserved in 100% ethanol. The samples were then dried for storage prior to further processing. This method is inexpensive and safe for long-distance transportation and at airports.
Abdalkaleq K. Suleman; Gulbahar F. Karim; Saad Dhamin Oleiwi; Karkaz M. Thalij
Volume 21, Issue 4 , December 2021, , Pages 159-167
Abstract
The Genomic DNA had been extracted from the liver tissue of the experimental rat groups following 15 weeks of treatment with partial purified L-glutaminase enzyme from E.coli in relation with ethylamine. The DNA samples of all the six treatment groups were amplified by PCR using three different primers ...
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The Genomic DNA had been extracted from the liver tissue of the experimental rat groups following 15 weeks of treatment with partial purified L-glutaminase enzyme from E.coli in relation with ethylamine. The DNA samples of all the six treatment groups were amplified by PCR using three different primers specific for determining the presence of P53, bax and G3pdh genes and detect the effect of Ethylenimine and L-glutaminase on the animals at the molecular level. The agarose gel electrophoresis technique used to analyze the PCR amplification products. The result revealed the presence of P53 gene in all treatment groups except for diseased group (T2). This finding demonstrates the mutagenic effect of Ethylenimine that lead to mutation at P53 gene sequence, and therapeutic beneficial of L-glutaminase. Also, there is no PCR amplification product which represent Bax gene sequence for T2 and T5 groups which were administered doses of Ethylenimine, indicating that low doses of L-glutaminase failed to prevent the mutagenic effect of Ethylenimine. While, the G3pd genes is presented only in T2 and T5. Finally, analysis the DNA sequences of the PCR amplified products extracted from liver samples of T2 group treated with Ethylenimine was carried out, then the results were compared with NCBI. The expected mutations were found at thirteen locals and there were only three mutated sequences with the DNA of liver samples from T5 group, while the DNA of animals in group (T6) were showed no mutated regions. The results of this novel study make clear the therapeutic effect of L-glutaminase and how suppress the mutagenic and carcinogenic effect of ethylenimine on P53, Bax, and G3pd genes, and its effect was dose dependent..
