Document Type : Articles
Authors
Food Sciences Dept., College of Agriculture, University of Tikrit, Tikrit, Iraq
Abstract
This study was conducted in at the Biotechnology Research Center / Nahrain University / Baghdad Governorate, for the period 2/8/2020 to 15/9/2020, the follow of the effects of dosing with ethanolic extract of pomegranate peels on 30 male rats of (2-3) months of age and weights (170-220 g) were included, it was divided into two parts, one of which is intact and the other in which diabetes was introduced by using alloxan at a concentration (90 mg / kg) of body weight, The results showed of the biological study that the development of experimental diabetes showed significant increase (P <0.05) in the concentrations of Glucose, Total cholesterol (TC), Trigleserid (TG), low-density lipoproteins cholesterol (LDL-C), Very-low-density lipoprotein cholesterol (VLDL-C), and the enzymes of aspartate transaminase and alanine transaminase (AST و ALT), and malonedialdehyde concentration (MDA), Compared with a intact control group, while it led to a significant decrease (P <0.05) in body weight, insulin concentration, high-density lipoprotein cholesterol (HDL), in the affected control group. Dosing in intact rats with ethanolic extract of pomegranate peel at a concentration of 75 and 150 mg / kg of body weight led to a significant decrease in the concentrations of glucose, TC, TG, LDL-C and VLDL-C, as well as AST, ALT, and MDA enzymes, compared with a healthy control group, Significant increase in body weight, insulin and HDL-C concentrations for all treatments compared with the healthy control group. Dosing of diabetic rats with ethanolic extract of pomegranate peels at a concentration of 75 and 150 mg / kg of body weight led to a significant decrease in the concentrations of glucose, TC, TG, LDL-C and VLDL-C, as well as AST, ALT, and MDA enzymes, compared with the infected and untreated control group, a significant increase in body weight, insulin and HDL-C concentrations, for all treatments, compared with the affected control group.
Keywords
- Abd El-Mageid, M. M., Salama, A. R., Saleh, M. A. M., and Abo-Taleb, H. N. (2016). Evaluation of antidiabetic, hypocholesterolemic of pomegranate (Punica granatum L.) juice powders and peel powder extracts in male albino rats. IOSR J. Pharm. Biol. Sci, 11: 53-64.
- Achi, N. K., Ohaeri, O. C., Ijeh, I. I., and Eleazu, C. (2017). Modulation of the lipid profile and insulin levels of streptozotocin induced diabetic rats by ethanol extract of Cnidoscolus aconitifolius leaves and some fractions: Effect on the oral glucose tolerance of normoglycemic rats. Biomedicine and Pharmacotherapy, 86, 562-569.
- Afify, A. E. M. M., El-Beltagi, H. S., Fayed, S. A., and El-Ansary, A. E. (2018). Enhancing effect of olive leaves extract on lipid profile and enzymes activity in streptozotocin induced diabetic rats. Blood, 1010(3.5), 13.
- Ahmed, M. A. (2012). Study the effect of vital component in Pomegranate fruit (Punica granatum) on some biochemical parameters in white rat males. Tikret Journal of Pharmaceutical Sciences, 8(1): 123-127.
- Al-Jafaar, S.H.N. (2020). Use of celery extract, pomegranate juice, and potassium supplements in the treatment of diabetic ketoacidosis in Alloxan induced in white male rats. Master Thesis, College of Agriculture, Tikrit University.
- Al-Badya, B.S.H (2018). The physiological, biochemical and histological effects of potato peels in male white rats. PhD thesis, College of Agriculture - Department of Food Sciences, University of Tikrit.
- Al-Bayati, M. N. H. (2017). Determination of the biochemical and histopathological effects of some diets in laboratory mice with induced by alloxan diabetes. Master Thesis, College of Agriculture, Tikrit University.
- Alhazza, I. M. (2007). Antioxidant and hypolipidemic effects of olive oil in normal and diabetic male rats. Faudi Journal of Biological Sciences; 14 (1): 69-74.
- Ali, S. A., Hamed, M. A., El-Rigal, N. S., Shabana, M. H., and Kassem, M. E. S. (2011). Chemical constituents of Argyreia speciosa Fam. Convolvulaceae and its role against hyperglycemia. Journal of Applied Pharmaceutical Science, 1(8): 76-84.
- Al-Jubouri, H. M.T. (2008). A study of the effect of aqueous extract of lemon plant and its comparison with vitamin C as antioxidants in male rats exposed to oxidative stress. Master Thesis, College of Education, University of Tikrit.
- Allain,C. C. (1974). Clinical Chemistray. 20/4, p: 470-475.
- Al-Muammar, M. N., and Khan, F. (2012). Obesity: the preventive role of the pomegranate (Punica granatum). Nutrition, 28(6): 595-604.
- Al-Muslehi, M. S. M. (2013). Effect of Powder of Pomegranate (Punica granatum) Peels on Lipid Profile in Hypercholesterolemic Rats. Kufa Journal For Veterinary Medical Sciences, 4(1): 111-117.
- Banihani, S., Swedan, S., and Alguraan, Z. (2013). Pomegranate and type 2 diabetes. Nutrition research, 33(5), 341-348.
- Betteridge, D. J. (2000). What is oxidative stress. Metabolism Clinical Experimental; 49(2): 1-5.
- Brewer Jr, H. B. (2004). Focus on high-density lipoproteins in reducing cardiovascular risk. American heart journal, 148(1): S14-S18.
- Burtis,C, A. and Ashwood, E. R. (1999). Teitz Text Book of Clinical Chemistry, 3rded., W.B. Saunders Company, London, UK., pp: 840-843.
- Dewanjee, S., Bose, S. K., Sahu, R., & Mandal, S. C. (2008). Antidiabetic effect of matured fruits of Diospyros peregrina in alloxan-induced diabetic rats. International Journal of Green Pharmacy (IJGP), 2(2):95-99.
- Duncan, P. and Mahaffey, (1994). Erythrocytes. Veterinary Laboratory Medicine, 3rd ed. Ames, Iowa state university press: 21-34
- El Mageid, M. M. A., Salama, N. A., Saleh, M. A. M., and Abo-Taleb, H. M. (2016). Evaluation of antdiabetic, hypocholesterolemic of pomegranate (Punica granatum L.) juice powders and peel powder extracts in male albino rats. IOSR-JPBS, 11(6): 53-64.
- El‐Hadary, A. E., and Ramadan, M. F. (2019). Phenolic profiles, antihyperglycemic, antihyperlipidemic, and antioxidant properties of pomegranate (Punica granatum) peel extract. Journal of food biochemistry, 43(4): 1–9.
- El-Missiry, M. A., and El Gindy, A. M. (2000). Amelioration of alloxan induced diabetes mellitus and oxidative stress in rats by oil of Eruca sativa seeds. Annals of Nutrition and Metabolism, 44(3): 97-100.
- Ene, A. C., Milala, M. A., and Nwankwo, E. A. (2006). The effect of different doses of black caraway (Carum carvi L.) oil on the liver enzymes of alloxan-induced diabetic rats. J Med Sci,6(6),994-8.
- Fossati, P. and Prencipe, L. (1982). Serum triglyceride determined calorimetrically with an enzyme that produce hydrogen per-oxide. Clin. Chem, 28(16): 2077-2080.
- Gabr, N. M. (2017). EFFECTS OF POMEGRANATE (PUNICA GRANATUM L.) FRESH JUICE AND PEEL EXTRACT ON DIABETIC MALE ALBINO RATS. Al-Azhar Medical Journal, 46(4): 965-980.
- Gao, W.G. (2010). Early detection of type 2 diabetes mellitus in chinese and Indian adult population. Diabet Med J., 27: 22-29.
- Guidet, B. and Shah, S. V. (1989). Am J. physiol, 257(26):440. cited by Muslih, R.K., Al-Nimer, M.S. and Al-Zamely, O.Y. (2002). The level of Malondialdehyde after activation with H2O2 and CuSO4 and inhibition by deferoxamine and Molsidomine in the serum of patient with acute Myocardial infarction. National J. of chemistry, 5:139-148.
- Gulfraz, M., Qadir, G., Nosheen, F., and Parveen, Z. (2007). Antihyperglycemic effects of Berberis lyceum Royle in alloxan induced diabetic rats. Diabetologia croatica, 36(3): 49-54.
- Hidayat, M., Tahir, M., and Shoro, A. A. (2014). Melatonin preserves the morphology of parotid gland damaged by streptozotocin induced diabetes. Journal of Postgraduate Medical Institute (Peshawar-Pakistan), 28(2):128-132.
- Ismaiil, L. A., Joumaa, W. H., and Moustafa, M. E. (2019). Selenium decreases triglycerides and VLDL-c in diabetic rats exposed to electromagnetic radiation from mobile phone base stations. Journal of Taibah University for Science, 13(1), 844-849.
- Kaleem, M., Medha, P., Ahmed, Q. U., Asif, M., and Bano, B. (2008). Beneficial effects of Annona squamosa extract in streptozotocin-induced diabetic rats. Singapore medical journal, 49(10), 800.
- Khateeb, J., Gantman, A., Kreitenberg, A. J., Aviram, M., and Fuhrman, B. (2010). Paraoxonase 1 (PON1) expression in hepatocytes is upregulated by pomegranate polyphenols: a role for PPAR-γ pathway. Atherosclerosis, 208(1):119-125.
- Lei, F., Zhang, X. N., Wang, W., Xing, D. M., Xie, W. D., Su, H., and Du, L. J. (2007). Evidence of anti-obesity effects of the pomegranate leaf extract in high-fat diet induced obese mice. International Journal of Obesity, 31(6): 1023-1029.
- Li, Y., Qi, Y., Huang, T. H., Yamahara, J., and Roufogalis, B. D. (2008). Pomegranate flower: a unique traditional antidiabetic medicine with dual PPAR‐α/‐γ activator properties. Diabetes, Obesity and Metabolism, 10(1): 10-17.
- Liu, J. (2005). Oleanolic acid and ursolic acid: research perspectives. J. Ethnopharmacol,100:92-94.
- Mans, K., and Aburjai, T. (2019). Accessing the hypoglycemic effects of seed extract from celery (Apium graveolens) in alloxan-induced diabetic rats. Journal of Pharmaceutical Research International, 1-10.
- Matthaeus, B. and Ozcan, M. (2016). Pomegranate plant (Punica granatum L.) composition, antioxidant activity, therapeutic effect and antimicrobial activity. Journal of Applied Pharmaceutical Science, 21: 160-167.
- Middha, S. K., Usha, T., and RaviKiran, T. (2012). Influence of Punica granatum L. on region specific responses in rat brain during Alloxan-Induced diabetes. Asian Pacific Journal of Tropical Biomedicine, 2(2): S905-S909.
- Middha, S.K., T. Usha and T. RaviKiran. (2012). Influence of Punica granatum L. on region specific responses in rat brain during alloxan-induced diabetes. Asian Pac. J. Trop. Biomed. 2(2):905-909.
- Mohammed, W. J., Sheet, W. M., Rahim, S. M., and Mohammed, M. J. (2011). Effect of effective microorganisms (EM) in blood sugar contrition and some biochemical parameters in normal and alloxan induced diabetic male rats. Rafidain Journal of Science, 22(4), 1-15.
- Moneim, A. E. A. (2012). Antioxidant activities of Punica granatum (pomegranate) peel extract on brain of rats. Journal of Medicinal Plants Research, 6(2): 195-199.
- Moneim, A. E. A., Dkhil, M. A., and Al-Quraishy, S. (2011). Studies on the effect of pomegranate (Punica granatum) juice and peel on liver and kidney in adult male rats. J Med Plants Res, 5(20), 5083-8.
- Mukhlif, Z. F., Rahim, S. M., and Jumaa Jamal, M. N. (2020). The Effect of Diabetes on the Histological Parameters in Alloxan Induced Diabetes Male Rats. Prensa Med Argent, 106, 6. 264.
- Murray R.K.; Granner, D.K.; Mayes. P.K.; and Rodwell V.W. (2000). Harper’s Biochemistry. 25th edition Appleton and Lange U.S.A: 551 -553.
- Nelson, D. L. and Cox, M. M. (2005). Lehninger Principles of Biochemistry. 4thed. W.H. Freeman and Company, New York. (18): 142–150.
- Oraki, H. H., Demirci, A. Ş., and Gümüş, T. (2011). Antibacterial and antifungal activity of pomegranate (Punica granatum L. cv.) peel. Electronic Journal of Environmental, Agricultural & Food Chemistry, 10(3).
- Osman, H. F., Eshak, M. G., El-Sherbiny, E. M., and Bayoumi, M. M. (2012). Biochemical and genetical evaluation of pomegranate impact on diabetes mellitus induced by alloxan in female rats. Life Sci J, 9(3): 1543-1553.
- Punasiya, R., Joshi, A., and Patidar, K. (2010). Antidiabetic Effect of an Aqueous Extract of Pomegranate (Punica granatum L.) Peels in Normal and Alloxan Diabetic Rats. Research Journal of Pharmacy and Technology, 3(1): 272-274.
- Ramzy, M. (2019). Role of pomegranate peel on ameliorated hyperglycemia and hypercholesterolemia in experimental rats. Journal of Medicine in Scientific Research, 2(3): 185.
- Rosenblat, M., and Aviram, M. (2009). Paraoxonases role in the prevention of cardiovascular diseases. Biofactors, 35(1): 98-104.
- Sadeghipour, A., Eidi, M., Ilchizadeh Kavgani, A., Ghahramani, R., Shahabzadeh, S., and Anissian, A. (2014). Lipid lowering effect of Punica granatum L. peel in high lipid diet fed male rats. Evidence-Based Complementary and Alternative Medicine, Article ID 432650.
- Sadia, H., Akter, Q. S., Afroz, R., and Siddika, T. (2016). Effect of Punica Granatum (Pomegranate) on serum ALT and AST in Carbon tetrachloride induced liver damage in Wistar Albino Rats. Journal of Bangladesh Society of Physiologist, 11(1), 23-28.
- Sakahar, D,; Sekahar, B,; Ranabir, S. and Subhash, M. (2008) Antidiabetic effect of matured fruits of Diospyros peregrine in alloxan induced diabetic rats. Int. J. gre. Pharm. 2(2):95-99.
- Salmenniemi, U., Ruotsalainen, E., Pihlajamäki, J., Vauhkonen, I., Kainulainen, S., Punnonen, K., and Laakso, M. (2004). Multiple abnormalities in glucose and energy metabolism and coordinated changes in levels of adiponectin, cytokines, and adhesion molecules in subjects with metabolic syndrome. Circulation, 110(25): 3842-3848.
- Salwe, K. J., Sachdev, D. O., Bahurupi, Y., and Kumarappan, M. (2015). Evaluation of antidiabetic, hypolipedimic and antioxidant activity of hydroalcoholic extract of leaves and fruit peel of Punica granatum in male Wistar albino rats. Journal of natural science, biology, and medicine, 6(1):56.
- SAS, (2001). SAS User s guide: Statistical system, Ine. Cary, NC. USA.
- Shaban, N. Z., El-Kersh, M. A., El-Rashidy, F. H., and Habashy, N. H. (2013). Protective role of Punica granatum (pomegranate) peel and seed oil extracts on diethylnitrosamine and phenobarbital-induced hepatic injury in male rats. Food chemistry, 141(3):1587-1596.
- Singh, N. and Gupta, M. (2007). Regeneration of cells in islets of Langerhans of pancreas of alloxan diabetic rats by acetone extract of Momordica charantia (Linn.) (bitter gourd) fruits. Indian J. Exp.Biology,45:1055- 1062.
- Suba, V., Murugesan, T., Arunachalam, G., Mandal, S. C., and Saha, B. P. (2004). Anti-diabetic potential of Barleria lupulina extract in rats. Phytomedicine, 11(2-3):202-205.
- Tietz, N. W. (1995). Clinical Guide to Laboratory Tests. 3rded. W.B. Saunders Company, Philadelphia, USA. pp. 266-273.
- Trinder, P. A. (1969). Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor. Ann. Clin. Bio chem, 6: 24-33.
- Vincent, M. E., and Desmond, B. A. (2018). Hypocholesterolemic Effect of Nauclea Latifolia Fruit on Glucose Changes and Lipid Profile of Alloxan Induced Diabetes in Albino Rats. International Journal of Advances in Scientific Research and Engineering, 4, 13-20.
- Xiao, L. (2003): Lipid Hydroperoxide (LOOH). Free Radical and Radiation Biol. Program. Unive. of lowa. Free radical in Biol.andMed.77:222.
- Zhang, L. (2010). Dyslipidemia, glucose intolerance and cardiovascular disease mortality and morbidity in Europeans and Asians. Academic dissertation. Publication of Public Health M205, University of Helsinki, Helsinki, Finland.